清華大學一篇論文，證明了動物細胞也具有全能性？_風聞

清華大學一篇論文，證明了動物細胞也具有全能性？

在自然雜誌的網站上，找到了up提到的這篇論文，標題為：Induction of mouse totipotent stem cells by a defined chemical cocktail，粗譯一下：通過確定的化學混合物誘導小鼠全能幹細胞

來看一下這篇文章的Abstract：

In mice, only the zygotes and blastomeres from 2-cell embryos are authentic totipotent stem cells (TotiSCs), capable of producing all the differentiated cells in both embryonic and extraembryonic tissues and forming an entire organism.

However, it remains challenging whether and how TotiSCs, representing the very beginning of a life, can be established in vitro in the absence of germline cells. Here, we demonstrate induction and long-term maintenance of TotiSCs from mouse pluripotent stem cells (PSCs) by a combination of three small molecules, TTNPB, 1-Azakenpaullone, and WS6. These cells, which we designated as ciTotiSCs (chemically induced totipotent stem cells), resembled mouse totipotent 2C-embryo stage cells at transcriptome, epigenome and metabolome level.

In addition, ciTotiSCs exhibited bidirectional developmental potentials and were able to produce both embryonic and extraembryonic cells in vitro and in teratoma. Furthermore, following injection into 8-cell embryo, ciTotiSCs contributed to both embryonic and extraembryonic lineages with high efficiency.

Our chemical approach for TotiSCs induction and maintenance provides a defined in vitro system to manipulate and understand totipotent state towards creating life from non-germline.

簡單機翻：

在小鼠中，只有來自 2 細胞胚胎的受精卵和卵裂球才是真正的全能幹細胞 (TotiSCs)，它們能夠在胚胎和胚胎外組織中產生所有分化的細胞並形成一個完整的有機體1。然而，在沒有生殖細胞的情況下，是否以及如何在體外建立代表生命開始的 TotiSCs 仍然具有挑戰性。

在這裏，我們展示了通過三種小分子 TTNPB、1-Azakenpaulllone 和 WS6 的組合對小鼠多能幹細胞 (PSCs) 的 TotiSCs 的誘導和長期維持。我們將這些細胞稱為 ciTotiSCs（化學誘導的全能幹細胞），在轉錄組、表觀基因組和代謝組水平上類似於小鼠全能 2C 胚胎期細胞。此外，ciTotiSCs 表現出雙向發育潛力，能夠在體外和畸胎瘤中產生胚胎和胚胎外細胞。

此外，在注射到 8 細胞胚胎中後，ciTotiSCs 對胚胎和胚胎外譜系都有高效的貢獻。我們用於 TotiSCs 誘導和維持的化學方法提供了一個明確的體外系統來操縱和理解全能狀態，從而從非生殖細胞中創造生命。

原文在此：https://www.nature.com/articles/s41586-022-04967-9#article-comments